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KMID : 0359319910310040397
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Korean Journal of Veterinary Research
1991 Volume.31 No. 4 p.397 ~ p.402
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Production of the standard antisera for blood typing of horses
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이시영/Lee, Shi Young
임영재/삼포신의/등정선이/무목일중/Lim, Young Jae/Miura, N ./S . Fujii/Mogi, K .
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Abstract
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The present experiments were undertaken to produce the standard antiserum for equine blood typing. The following results were obtained through ISO and Hetero Immunizations of the horses whose blood typing was analysed in the Laboratory of Racing Chemistry of Japan.
1. Of the 21 combinations of ISO-immune, 17 horses were produced antibody (about 80%)
2. Antibody titers were increased from early 1 week to late 5 weeks and any antibody titers were not be obtained in spite of the using of adjuvant and 10 repeated injections in the other 4 horses.
3. High antibody titers were obtained within the earliest period in the Dd antigen but were not increased over 32 times in spite of 8∼10 repeated injections in the antigen.
4. Antibody were easily produced in the Ca antigen of ISO-Immune but production of antisera were tailed due to abscence of absorbed blood cell.
5. Antibody titers of 1,024 times were obtained through 5 injections in the Ca of Hetero Immune
6. Of the produced 15 antisera (16 system), 13 antigen (5 system) were absorbed.
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